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Synthetic Equine β Endorphin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β endorphin  (Biorbyt)
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The effect of GLP-1RA on IL-10 and <t>β-endorphin</t> in paclitaxel-treated 50B11 DRG neuronal cell line. (A) QPCR analysis for IL-10 mRNA level and ELISA assay for secreted IL-10. IL-10 mRNA expression and secreted IL-10 were significantly decreased by paclitaxel, which was restored by GLP-1RA in the 10 ng/mL concentration. (B) QPCR analysis and immunofluorescence assay of POMC and β-endorphin. (C) QPCR analysis and immunofluorescence assay of μ-opioid receptor. Data are shown as mean ± standard deviation. n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar = 100 um. FSK: Forskolin, PAC: Forskolin + Paclitaxel, G0.01: Forskolin + Paclitaxel + GLP-1RA 0.01 µg/mL (treated 24 hr after paclitaxel treatment), G0.1: Forskolin + Paclitaxel + GLP-1RA 0.1 µg/mL (treated 24 hr after paclitaxel treatment), G1: Forskolin + Paclitaxel + GLP-1RA 1 µg/mL (treated 24 hr after paclitaxel treatment), G10: Forskolin + Paclitaxel + GLP-1RA 10 µg/mL (treated 24 hr after paclitaxel treatment), GLP-1RA: glucagon like peptide-1 receptor agonist, IL-10: interleukin-10, DRG: dorsal root ganglion, POMC: pro-opiomelanocortin, MOR: μ-opioid receptor, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.
β Endorphin, supplied by Biorbyt, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human β-endorphin elisa kit-colorimetric nbp2-78774  (Novus Biologicals)
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The effect of GLP-1RA on IL-10 and <t>β-endorphin</t> in paclitaxel-treated 50B11 DRG neuronal cell line. (A) QPCR analysis for IL-10 mRNA level and ELISA assay for secreted IL-10. IL-10 mRNA expression and secreted IL-10 were significantly decreased by paclitaxel, which was restored by GLP-1RA in the 10 ng/mL concentration. (B) QPCR analysis and immunofluorescence assay of POMC and β-endorphin. (C) QPCR analysis and immunofluorescence assay of μ-opioid receptor. Data are shown as mean ± standard deviation. n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar = 100 um. FSK: Forskolin, PAC: Forskolin + Paclitaxel, G0.01: Forskolin + Paclitaxel + GLP-1RA 0.01 µg/mL (treated 24 hr after paclitaxel treatment), G0.1: Forskolin + Paclitaxel + GLP-1RA 0.1 µg/mL (treated 24 hr after paclitaxel treatment), G1: Forskolin + Paclitaxel + GLP-1RA 1 µg/mL (treated 24 hr after paclitaxel treatment), G10: Forskolin + Paclitaxel + GLP-1RA 10 µg/mL (treated 24 hr after paclitaxel treatment), GLP-1RA: glucagon like peptide-1 receptor agonist, IL-10: interleukin-10, DRG: dorsal root ganglion, POMC: pro-opiomelanocortin, MOR: μ-opioid receptor, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.
Human β Endorphin Elisa Kit Colorimetric Nbp2 78774, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monomeric β-endorphin chinapeptides-qyaobio  (ChinaPeptides)
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The effect of GLP-1RA on IL-10 and <t>β-endorphin</t> in paclitaxel-treated 50B11 DRG neuronal cell line. (A) QPCR analysis for IL-10 mRNA level and ELISA assay for secreted IL-10. IL-10 mRNA expression and secreted IL-10 were significantly decreased by paclitaxel, which was restored by GLP-1RA in the 10 ng/mL concentration. (B) QPCR analysis and immunofluorescence assay of POMC and β-endorphin. (C) QPCR analysis and immunofluorescence assay of μ-opioid receptor. Data are shown as mean ± standard deviation. n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar = 100 um. FSK: Forskolin, PAC: Forskolin + Paclitaxel, G0.01: Forskolin + Paclitaxel + GLP-1RA 0.01 µg/mL (treated 24 hr after paclitaxel treatment), G0.1: Forskolin + Paclitaxel + GLP-1RA 0.1 µg/mL (treated 24 hr after paclitaxel treatment), G1: Forskolin + Paclitaxel + GLP-1RA 1 µg/mL (treated 24 hr after paclitaxel treatment), G10: Forskolin + Paclitaxel + GLP-1RA 10 µg/mL (treated 24 hr after paclitaxel treatment), GLP-1RA: glucagon like peptide-1 receptor agonist, IL-10: interleukin-10, DRG: dorsal root ganglion, POMC: pro-opiomelanocortin, MOR: μ-opioid receptor, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.
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The effect of GLP-1RA on IL-10 and <t>β-endorphin</t> in paclitaxel-treated 50B11 DRG neuronal cell line. (A) QPCR analysis for IL-10 mRNA level and ELISA assay for secreted IL-10. IL-10 mRNA expression and secreted IL-10 were significantly decreased by paclitaxel, which was restored by GLP-1RA in the 10 ng/mL concentration. (B) QPCR analysis and immunofluorescence assay of POMC and β-endorphin. (C) QPCR analysis and immunofluorescence assay of μ-opioid receptor. Data are shown as mean ± standard deviation. n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar = 100 um. FSK: Forskolin, PAC: Forskolin + Paclitaxel, G0.01: Forskolin + Paclitaxel + GLP-1RA 0.01 µg/mL (treated 24 hr after paclitaxel treatment), G0.1: Forskolin + Paclitaxel + GLP-1RA 0.1 µg/mL (treated 24 hr after paclitaxel treatment), G1: Forskolin + Paclitaxel + GLP-1RA 1 µg/mL (treated 24 hr after paclitaxel treatment), G10: Forskolin + Paclitaxel + GLP-1RA 10 µg/mL (treated 24 hr after paclitaxel treatment), GLP-1RA: glucagon like peptide-1 receptor agonist, IL-10: interleukin-10, DRG: dorsal root ganglion, POMC: pro-opiomelanocortin, MOR: μ-opioid receptor, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.
Human β Endorphin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The effect of GLP-1RA on IL-10 and <t>β-endorphin</t> in paclitaxel-treated 50B11 DRG neuronal cell line. (A) QPCR analysis for IL-10 mRNA level and ELISA assay for secreted IL-10. IL-10 mRNA expression and secreted IL-10 were significantly decreased by paclitaxel, which was restored by GLP-1RA in the 10 ng/mL concentration. (B) QPCR analysis and immunofluorescence assay of POMC and β-endorphin. (C) QPCR analysis and immunofluorescence assay of μ-opioid receptor. Data are shown as mean ± standard deviation. n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar = 100 um. FSK: Forskolin, PAC: Forskolin + Paclitaxel, G0.01: Forskolin + Paclitaxel + GLP-1RA 0.01 µg/mL (treated 24 hr after paclitaxel treatment), G0.1: Forskolin + Paclitaxel + GLP-1RA 0.1 µg/mL (treated 24 hr after paclitaxel treatment), G1: Forskolin + Paclitaxel + GLP-1RA 1 µg/mL (treated 24 hr after paclitaxel treatment), G10: Forskolin + Paclitaxel + GLP-1RA 10 µg/mL (treated 24 hr after paclitaxel treatment), GLP-1RA: glucagon like peptide-1 receptor agonist, IL-10: interleukin-10, DRG: dorsal root ganglion, POMC: pro-opiomelanocortin, MOR: μ-opioid receptor, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.
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human-ep (β-endorphin) elisa kit  (Elabscience Biotechnology)
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The effect of GLP-1RA on IL-10 and <t>β-endorphin</t> in paclitaxel-treated 50B11 DRG neuronal cell line. (A) QPCR analysis for IL-10 mRNA level and ELISA assay for secreted IL-10. IL-10 mRNA expression and secreted IL-10 were significantly decreased by paclitaxel, which was restored by GLP-1RA in the 10 ng/mL concentration. (B) QPCR analysis and immunofluorescence assay of POMC and β-endorphin. (C) QPCR analysis and immunofluorescence assay of μ-opioid receptor. Data are shown as mean ± standard deviation. n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar = 100 um. FSK: Forskolin, PAC: Forskolin + Paclitaxel, G0.01: Forskolin + Paclitaxel + GLP-1RA 0.01 µg/mL (treated 24 hr after paclitaxel treatment), G0.1: Forskolin + Paclitaxel + GLP-1RA 0.1 µg/mL (treated 24 hr after paclitaxel treatment), G1: Forskolin + Paclitaxel + GLP-1RA 1 µg/mL (treated 24 hr after paclitaxel treatment), G10: Forskolin + Paclitaxel + GLP-1RA 10 µg/mL (treated 24 hr after paclitaxel treatment), GLP-1RA: glucagon like peptide-1 receptor agonist, IL-10: interleukin-10, DRG: dorsal root ganglion, POMC: pro-opiomelanocortin, MOR: μ-opioid receptor, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.
Human Ep (β Endorphin) Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β-endorphin  (Biologica Environmental Services)
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Biologica Environmental Services β-endorphin
The effect of GLP-1RA on IL-10 and <t>β-endorphin</t> in paclitaxel-treated 50B11 DRG neuronal cell line. (A) QPCR analysis for IL-10 mRNA level and ELISA assay for secreted IL-10. IL-10 mRNA expression and secreted IL-10 were significantly decreased by paclitaxel, which was restored by GLP-1RA in the 10 ng/mL concentration. (B) QPCR analysis and immunofluorescence assay of POMC and β-endorphin. (C) QPCR analysis and immunofluorescence assay of μ-opioid receptor. Data are shown as mean ± standard deviation. n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar = 100 um. FSK: Forskolin, PAC: Forskolin + Paclitaxel, G0.01: Forskolin + Paclitaxel + GLP-1RA 0.01 µg/mL (treated 24 hr after paclitaxel treatment), G0.1: Forskolin + Paclitaxel + GLP-1RA 0.1 µg/mL (treated 24 hr after paclitaxel treatment), G1: Forskolin + Paclitaxel + GLP-1RA 1 µg/mL (treated 24 hr after paclitaxel treatment), G10: Forskolin + Paclitaxel + GLP-1RA 10 µg/mL (treated 24 hr after paclitaxel treatment), GLP-1RA: glucagon like peptide-1 receptor agonist, IL-10: interleukin-10, DRG: dorsal root ganglion, POMC: pro-opiomelanocortin, MOR: μ-opioid receptor, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.
β Endorphin, supplied by Biologica Environmental Services, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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β-endorphin d731172  (Sangon Biotech)
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Sangon Biotech β-endorphin d731172
The effect of GLP-1RA on IL-10 and <t>β-endorphin</t> in paclitaxel-treated 50B11 DRG neuronal cell line. (A) QPCR analysis for IL-10 mRNA level and ELISA assay for secreted IL-10. IL-10 mRNA expression and secreted IL-10 were significantly decreased by paclitaxel, which was restored by GLP-1RA in the 10 ng/mL concentration. (B) QPCR analysis and immunofluorescence assay of POMC and β-endorphin. (C) QPCR analysis and immunofluorescence assay of μ-opioid receptor. Data are shown as mean ± standard deviation. n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar = 100 um. FSK: Forskolin, PAC: Forskolin + Paclitaxel, G0.01: Forskolin + Paclitaxel + GLP-1RA 0.01 µg/mL (treated 24 hr after paclitaxel treatment), G0.1: Forskolin + Paclitaxel + GLP-1RA 0.1 µg/mL (treated 24 hr after paclitaxel treatment), G1: Forskolin + Paclitaxel + GLP-1RA 1 µg/mL (treated 24 hr after paclitaxel treatment), G10: Forskolin + Paclitaxel + GLP-1RA 10 µg/mL (treated 24 hr after paclitaxel treatment), GLP-1RA: glucagon like peptide-1 receptor agonist, IL-10: interleukin-10, DRG: dorsal root ganglion, POMC: pro-opiomelanocortin, MOR: μ-opioid receptor, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.
β Endorphin D731172, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The effect of GLP-1RA on IL-10 and β-endorphin in paclitaxel-treated 50B11 DRG neuronal cell line. (A) QPCR analysis for IL-10 mRNA level and ELISA assay for secreted IL-10. IL-10 mRNA expression and secreted IL-10 were significantly decreased by paclitaxel, which was restored by GLP-1RA in the 10 ng/mL concentration. (B) QPCR analysis and immunofluorescence assay of POMC and β-endorphin. (C) QPCR analysis and immunofluorescence assay of μ-opioid receptor. Data are shown as mean ± standard deviation. n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar = 100 um. FSK: Forskolin, PAC: Forskolin + Paclitaxel, G0.01: Forskolin + Paclitaxel + GLP-1RA 0.01 µg/mL (treated 24 hr after paclitaxel treatment), G0.1: Forskolin + Paclitaxel + GLP-1RA 0.1 µg/mL (treated 24 hr after paclitaxel treatment), G1: Forskolin + Paclitaxel + GLP-1RA 1 µg/mL (treated 24 hr after paclitaxel treatment), G10: Forskolin + Paclitaxel + GLP-1RA 10 µg/mL (treated 24 hr after paclitaxel treatment), GLP-1RA: glucagon like peptide-1 receptor agonist, IL-10: interleukin-10, DRG: dorsal root ganglion, POMC: pro-opiomelanocortin, MOR: μ-opioid receptor, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.

Journal: The Korean Journal of Pain

Article Title: Effect of glucagon-like peptide-1 receptor agonist on paclitaxel induced neurotoxicity in dorsal root ganglion neuronal cells in vitro

doi: 10.3344/kjp.24419

Figure Lengend Snippet: The effect of GLP-1RA on IL-10 and β-endorphin in paclitaxel-treated 50B11 DRG neuronal cell line. (A) QPCR analysis for IL-10 mRNA level and ELISA assay for secreted IL-10. IL-10 mRNA expression and secreted IL-10 were significantly decreased by paclitaxel, which was restored by GLP-1RA in the 10 ng/mL concentration. (B) QPCR analysis and immunofluorescence assay of POMC and β-endorphin. (C) QPCR analysis and immunofluorescence assay of μ-opioid receptor. Data are shown as mean ± standard deviation. n = 3. * P < 0.05, ** P < 0.01, *** P < 0.001. Scale bar = 100 um. FSK: Forskolin, PAC: Forskolin + Paclitaxel, G0.01: Forskolin + Paclitaxel + GLP-1RA 0.01 µg/mL (treated 24 hr after paclitaxel treatment), G0.1: Forskolin + Paclitaxel + GLP-1RA 0.1 µg/mL (treated 24 hr after paclitaxel treatment), G1: Forskolin + Paclitaxel + GLP-1RA 1 µg/mL (treated 24 hr after paclitaxel treatment), G10: Forskolin + Paclitaxel + GLP-1RA 10 µg/mL (treated 24 hr after paclitaxel treatment), GLP-1RA: glucagon like peptide-1 receptor agonist, IL-10: interleukin-10, DRG: dorsal root ganglion, POMC: pro-opiomelanocortin, MOR: μ-opioid receptor, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: The coverslips were incubated overnight at 4°C with primary antibodies targeting the μ opioid receptor (Thermo Fisher Scientific), β-endorphin (Biorbyt Ltd.), and βIII tubulin (Abclonal Biotechnology Co., Ltd.).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Concentration Assay, Immunofluorescence, Standard Deviation